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#dotblot #RohitMane #ScientistRacademy Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Due to the simplicity of the technique it widely used as a ideal diagnostic tool. This post covers the Protein Dot Blot Technique. Dot Blot Principle Dot Blot works based on the immunodetection principle for identifying specific protein. Dot Blot - PrincipleDot Blot - Technique: Overview Proteins are immobilized on to a protein binding membrane, PVDF or Nitrocellulose. After Protein Immobilization membrane is blocked to avoid non-specific binding, using blocking buffer. Immobilized protein is then incubated with a primary antibody (for 30mins to 1 hour). The antibody is specific to the protein and is visualized either with a specific tag or with a secondary antibody. The secondary antibody generally will carry a tag that allow the visualization of protein. Blots can be visualized using methods such as Chemiluminescence or Fluorescence. Common Tags used in Dot Blots are enzymes conjugated to the secondary antibody. Upon addition of substrate, enzyme acts on the substrate to develop colour. Commonly preferred enzymes are HRP(Horse Radish Peroxidase) and Alkaline Phosphatase (AP) Advantages of Dot Blot over Western Blot Non-Electrophoresis Technique: Dot Blot is non-electrophoresis technique, means that one need not do protein electrophoresis. Proteins can directly immobilized on to the membrane. Simple & Easy Method: No messy work of PAGE run and gel to membrane transfers, proteins are directly immobilized on membrane, making this method much easier and faster. Time: It is a faster method, takes ~3hours, whereas western blot may even take up to 2 days. Including the protein electrophoresis and gel to membrane transfer optimization. Dot Blot is not as informative as Western Blot, but it is inexpesive, quick and easy method for the simple protein detection. dot blot technique by shomus biology dot blot technique in hindi

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Luxor Las Vegas is a 30-story (106.7 meters tall) casino hotel situated on the southern end of the Las Vegas Strip in Paradise, Nevada. The hotel is owned by Vici Properties and operated by MGM Resorts International and has a 120,000-square-foot (11,000 m2) casino with over 2,000 slot machines and 87 table games. The casino opened in 1993 and was renovated and expanded several times. Later renovation work modernized the design of the property and raised the hotel's capacity to 4,407 rooms, including 442 suites. The hotel's rooms line the interior walls of the main tower, which has a pyramid shape, and the 22-story twin ziggurat towers. The hotel is named for the city of Luxor (ancient Thebes) in Egypt. The resort was announced by Circus Circus Enterprises on November 14, 1991. Known temporarily as "Project X", the pyramid-shaped resort would cost $290 million, and would be built on the Las Vegas Strip, on land located south of the company's Excalibur Hotel and Casino. Groundbreaking took place on April 21, 1992, with the project then known as "Luxor", after the Egyptian city of the same name. Veldon Simpson was the architect, and Perini Building Company was the general contractor. Waltek, a Cincinnati-based company, provided the metal-and-glass exterior for the pyramid. Standing 30 stories high, the pyramid was one of the largest metal-and-glass projects ever. The pyramid was topped off on July 9, 1993. The Luxor would compete against two other upcoming resorts, MGM Grand and Treasure Island. All three resorts had a family-oriented focus. The resort officially opened at 4 a.m. on October 15, 1993, to a crowd of 10,000 people. When it opened, the pyramid, which cost $375 million to build, was the tallest building on the strip and contained 2,526 rooms and a 100,000 sq ft (9,300 m2) casino. The resort was financed by "petty cash" earned from other Circus Circus Enterprises properties and did not include any outside financial investors. The hotel's pyramid is similar in size to the Red Pyramid and Bent Pyramid of Egypt. When the hotel opened, it was the tallest structure on the Strip, surpassed after only 11 days by Treasure Island, and featured the world's largest atrium, which has since been surpassed.

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Bonanza is an American Western television series that ran on NBC from September 12, 1959, to January 16, 1973. Lasting 14 seasons and 431 episodes, Bonanza is NBC's longest-running western, the second-longest-running western series on U.S. network television (behind CBS's Gunsmoke), and within the top 10 longest-running, live-action American series. The show continues to air in syndication. The show is set in the 1860s and centers on the wealthy Cartwright family, who live in the vicinity of Virginia City, Nevada, bordering Lake Tahoe. The series initially starred Lorne Greene, Pernell Roberts, Dan Blocker and Michael Landon and later featured (at various times) Guy Williams, David Canary, Mitch Vogel and Tim Matheson. The show is known for presenting pressing moral dilemmas.[2] The title "Bonanza" is a term used by miners in regard to a large vein or deposit of silver ore,[3] from Spanish bonanza (prosperity) and commonly refers to the 1859 revelation of the Comstock Lode of rich silver ore mines under the town of Virginia City, not far from the fictional Ponderosa Ranch that the Cartwright family operated. The show's theme song, also titled "Bonanza", became a hit song. Only instrumental renditions, absent Ray Evans' lyrics, were used during the series's long run.[4] In 2002, Bonanza was ranked No. 43 on TV Guide's 50 Greatest TV Shows of All Time,[5] and in 2013 TV Guide included it in its list of The 60 Greatest Dramas of All Time.[6] The time period for the television series is roughly between 1861 (Season 1) and 1867 (Season 13) during and shortly after the American Civil War, coinciding with the period Nevada Territory became a U.S. state. During the summer of 1972, NBC aired reruns of episodes from the 1967–1970 period in prime time on Tuesday evening under the title Ponderosa.[

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