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Comprehensive coverage and analysis of Union Budget 2018-19. Date: February 01, 2018 Challenges to FM Achievements & Objectives Guest - Shri Nitin Jairam Gadkari, Minister for Road Transport and Highways & Shipping, Govt of India, Dhanendra Kumar (VIF), Former Executive Director for India in the World Bank Washington, Former Chairman,Competition Commission of India (CCI), Dr. Shamika Ravi, Member, Economic Advisory Council to the Prime Minister,Mr Vijay Thadani, Vice Chairman & Managing Director, NIIT Limited Anchor: Pranjal Sharma

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#dotblot #RohitMane #ScientistRacademy Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Due to the simplicity of the technique it widely used as a ideal diagnostic tool. This post covers the Protein Dot Blot Technique. Dot Blot Principle Dot Blot works based on the immunodetection principle for identifying specific protein. Dot Blot - PrincipleDot Blot - Technique: Overview Proteins are immobilized on to a protein binding membrane, PVDF or Nitrocellulose. After Protein Immobilization membrane is blocked to avoid non-specific binding, using blocking buffer. Immobilized protein is then incubated with a primary antibody (for 30mins to 1 hour). The antibody is specific to the protein and is visualized either with a specific tag or with a secondary antibody. The secondary antibody generally will carry a tag that allow the visualization of protein. Blots can be visualized using methods such as Chemiluminescence or Fluorescence. Common Tags used in Dot Blots are enzymes conjugated to the secondary antibody. Upon addition of substrate, enzyme acts on the substrate to develop colour. Commonly preferred enzymes are HRP(Horse Radish Peroxidase) and Alkaline Phosphatase (AP) Advantages of Dot Blot over Western Blot Non-Electrophoresis Technique: Dot Blot is non-electrophoresis technique, means that one need not do protein electrophoresis. Proteins can directly immobilized on to the membrane. Simple & Easy Method: No messy work of PAGE run and gel to membrane transfers, proteins are directly immobilized on membrane, making this method much easier and faster. Time: It is a faster method, takes ~3hours, whereas western blot may even take up to 2 days. Including the protein electrophoresis and gel to membrane transfer optimization. Dot Blot is not as informative as Western Blot, but it is inexpesive, quick and easy method for the simple protein detection. dot blot technique by shomus biology dot blot technique in hindi

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La máquina ideal para realizar el enrollado del extremo de las barras macizas en forma de caracol. La Máquina de forjar puntas en caliente Nargesa PC16, facilita el trabajo de enrollado del hierro en caliente. Moldea la punta forjada en forma de caracol. Estas decoraciones son muy utilizadas como terminaciones en volutas y piezas decorativas. Rejas, ventanas, vallados, muebles metálicos etc... #Forjar #MaquinaForjar #Nargesa ¡Suscríbete al canal de YouTube de Nargesa! https://bit.ly/Nargesa Más videos relacionados http://bit.ly/NargesaLista CURVADORAS DE TUBOS/DOBLADORAS DE CAÑOS http://bit.ly/CurvadorasdeTubos Siguenos en nuestras redes sociales: Web: https://www.nargesa.com/ Facebook: https://www.facebook.com/prada.nargesa Instagram: https://www.instagram.com/prada.nargesa/ Twitter: https://twitter.com/PradaNargesaSL Pinterest: https://www.pinterest.es/nargesamaquinaria Linkedin: https://www.linkedin.com/company/prada-nargesa-sl/ ¡Bienvenido al canal de YouTube de Nargesa! Empresa de fabricación y venta de maquinaria industrial para la transformación del hierro en frío y en caliente Aquí encontrarás todo el funcionamiento de nuestra maquinaria de industria, forja artística y decorativa. Ajuste de recorrido o vueltas. Ajuste de presión según espesor del material. Bimando de seguridad para el accionamiento del ciclo. Mordaza hidráulica para la sujeción de la pieza. Capacidad máxima en redondo: 20mm Capacidad máxima en cuadrado: 20mm Capacidad máxima en pletina o planchuela: 50x10mm Velocidad de trabajo: 45 mm/s Velocidad de retroceso: 70 mm/s Tiempo de ciclo: 10 segundos Piezas por minuto: 4 piezas. Potencia de motor: 2,2 KW/ 3 CV (HP) Tensión 3 fases: 230/400 V Para otras tensiones o máquinas monofásicas consultar con el fabricante. Bancada inferior paletizada para poder transportar fácilmente la máquina al lugar de trabajo o almacenar. La máquina se envía completamente montada. Embalaje con palet de madera NIMF15 y caja de cartón de 3 capas apta para el transporte marítimo. Opcional: embalaje completo de madera fumigada NIMF15. VENTAJAS: Facilidad de uso: Cualquier operario es capaz de realizar el trabajo sin ningún tipo de preparación. Rapidez: Pueden realizarse miles de piezas en una sola jornada laboral. Valor y prestigio: Actualmente quedan pocos artesanos que puedan realizar estos trabajos forjados. Venta: posibilidad de vender piezas con las puntas forjadas a diferentes empresas o almacenes de hierro. +INFO http://www.nargesa.com/es/maquinaria-industrial/maquina-de-forja-en-caliente-pc16 Prada Nargesa S.L. Fabricación y venta de maquinaria industrial [email protected] Tel. (+34) 972568085 http://www.nargesa.com

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Morongo Casino, Resort & Spa is a Native American gaming casino, of the Morongo Band of Cahuilla Mission Indians, located in Cabazon, California, United States, near San Gorgonio Pass. The casino has 310 rooms and suites. A 44-acre (180,000 m2), 27-story resort, Morongo is one of the largest casinos in California. At 330 feet (101 m) high, the casino tower is the tallest building in both Riverside County and the larger Inland Empire region. Morongo is one of only six AAA four-diamond casino resorts in California, with Barona Casino, Viejas Casino, Pechanga Resort and Casino, Thunder Valley Casino Resort and the Chumash Casino Resort being the others. Morongo's main casino floor is 148,000 square feet (14,000 m2) with over 2,000 slots and table games. Upon entering the casino, guests see a contemporary, retro-1960s interior. Light coves between overhead vaults, back-lit fabric draped between frames, and conically shaped light fixtures create a complex, ornate ceiling above the casino floor. The casino offers more than a hundred table games, including four-card poker, Ultimate Texas Hold'Em, Mystery Card Roulette, blackjack, pai gow poker, mini-baccarat, and three card poker, as well as a 22-table poker room. High rollers can use a high-limit slot area with four table games and seven slots. The high-limit room also has a lounge with plasma TVs and complimentary food. Morongo's hotel accommodates guests in 272 standard rooms, 32 double-bay suites, and six casitas, the latter of which are located on an upper level surrounding the pool. Morongo's restaurants include the penthouse restaurant Cielo, Serrano (24-Hour Cafe), Tacos & Tequila, Sunset Bar & Grill, Natural 9 Noodle Company, and the Potrero Canyon Buffet, as well as a food court. Guests must be 18 or older to gamble on the property. - Wikipedia Let me know if you would like another casino walk through? #walkingtour #indiancasino #handpay #walking #bubblecraps #electroniccraps #tablecraps #betting #etg #slotplay #winning #casino #casinogame #casinoonline #casinogames #casinoonlineslot #slotmachine #lasvegas #gambling #gambler #gamblers #gaming #betting #onlinebet #wager #dragonlink #dragonlinkslotmachine #reno #nevada #shortvideo #funnyvideo #funnyvideo #viral #vegas #vegasgaming #comedy #fyp #fypviral #circuscircus #blackjack #craps #bubblecraps #roulette #etg200k #cardgame #cardgames #betting #wager #casinofloor #casinofun #craps #bubblecraps #compulsive #compulsivegambler #slotstrategy #ceasarspalace #palazzo #wynn #encorecasino #venetianlasvegas #mgm #roulette #baccarat